Professor

Tel: 0510-85197760

Email: bioyuxw@aliyun.com

Web: http://jndx.wshoto.com/

Oct.,   2013-Present          Professor,   Jiangnan University

July,2013-Aug.,2013           Visiting scientist, the State University of New Jersey, USA

Jan., 2011-Dec.,   2011        Visiting scientist, Swiss   Federal Institute of Technology ZurichJune, 2006-Oct., 2013            Associate   professor, Jiangnan University

Sept., 2001-June,   2006       Ph.D. in Biochemistry and molecular   biology

                           Zhejiang University, College of Life Science, P.R.China

Sept., 1997-July, 2001               Bachelor’s degree in Biochemistry and molecular biology 

                         Zhejiang   University, College of Life Science, P.R.China

1)         Enzyme discovery and   evolution. To discover novel industrial enzymes, such as lipase, protease;   Using directed evolution or rational design to improve enzymatic properties

2)       Enzyme structure and mechanism. Using   X-ray and NMR to solve the structure of lipase and study the interfacial   activation of lipase

3)         Protein expression. Mechanism   of high level expression of recombinant proteins in P. pastoris by   transcriptomics; Engineering of thermostable P. pastoris; Optimization   of fermentation process

1.           Xiao-Wei Yu,   Yan Xu, Rong Xiao. Lipases from   the genus Rhizopus: Characteristics, expression, protein   engineering and application. Progress in Lipid Research   2016,64:57–68 (IF=10.583)

2.           Xiao-Wei Yu , Min Yang, Chuanhuan Jiang, Xiaofeng Zhang, Yan Xu,   N-Glycosylation engineering to improve the constitutive expression of Rhizopus oryzae lipase in Komagataella phaffii,   Journal of Agricultural and   Food Chemistry, 2017,   65:6009-6015 (IF=3.154)

3.           Xiao-Wei Yu, Chong Sha, Yong-Liang Guo, Rong Xiao, Yan Xu, High-level expression and characterization of   a chimeric lipase from Rhizopus oryzae for biodiesel production, Biotechnology   for Biofuels 2013,6:29 (IF 5.203)

4.           Xiao-Wei Yu, Shan-Shan Zhu, Rong Xiao, Yan Xu. Conversion   of a Rhizopus chinensis lipase into an esterase by lid swapping. Journal   of Lipid Research, 2014,55:1044-1051   (IF 4.810)

5.           Meng Zhang, Xiao-Wei Yu*, Yan Xu*, Paula   Jouhten, G. V. T. Swapna, Ralf W. Glaser, John F Hunt, Gaetano T. Montelione,   Hannu Maaheimo and Thomas Szyperski. ¹³C metabolic flux profiling   of Pichia pastoris grown in aerobic batch cultures on glucose revealed   high relative anabolic use of TCA cycle and limited incorporation of provided   precursors of branched-chain amino acids. The FEBS Journal. 2017,   284:3100-3113 (IF: 3.902)

6.           Meng Zhang, Xiao‑Wei Yu*, G. V. T. Swapna, Rong Xiao, Haiyan Zheng, Chong Sha, Yan Xu*,   Gaetano T. Montelione. Efficient production of ²H, ¹³C,   ¹⁵N-enriched industrial enzyme Rhizopus chinensis lipase   with native disulfide bonds. Microbial Cell   Factories 2016,15:123 (IF3.681)

7.           Min   Yang, Xiao-Wei Yu*, H Y Zheng, C Sha, et al. Role   of N-linked glycosylation in the secretion and enzymatic properties of Rhizopus   chinensis lipase expressed in Pichia pastoris. Microbial Cell Factories,   2015,14:40-54. (IF3.681)

8.           Xiao-Wei Yu, Rui Wang, Yan Xu, Rong Xiao   and Meng Zhang, Enhanced thermostability of a Rhizopus chinensis lipase   by in vivo recombine tion in Pichia pastoris. Microbial Cell   Factories 2012,11:102-112 (IF3.681)

1.                National Natural Science Foundation of   China(NSFC) “Molecular recognition mechanism and function regulation of   lipase on the specific catalysis of structured lipids” No. 31671799,   2017~2020

2.                National High-tech Program (863) “Enzyme and   its application in food industry” No. 2012AA022207,   2012~2015

3.                National High-tech   Program (863) “Direct evolution and over-expression of the lipase from Burkholderia cepacia for highly enantioselective hydrolysis   of dl-menthyl acetate to l-menthol” No. 2008AA10Z304, 2008～2011

4.                National Natural   Science Foundation of China(NSFC) “Mechanism of microbiology enzyme   esterification based on the enzyme activity finger print” No.20802027, 2009～2011