Long Liu

Professor

Tel:+86-510-85918312

Email:longliu@jiangnan.edu.cn

Web:http://en.biotech.jiangnan.edu.cn/info/1034/1138.htm

2014 - Present Professor, School of Biotechnology, Jiangnan University

2012 - 2013 Postdoctoral Fellow, Georgia Institute of Technology

2010 - 2014 Associate Professor, School of Biotechnology, Jiangnan University

2009 - 2010 Lecturer, School of Biotechnology, Jiangnan University

2004 - 2009 Ph.D, Jiangnan University

2000 - 2004 Bachelor, Yantai University

The research fields include the rational design of the chassis cells of model industrial microorganisms, the construction of complex gene circuits and the research of dynamic metabolic engineering by using synthetic biology technology, including the excavation and creation of metabolic elements, the design and optimization of metabolic pathways, the modeling and reconstruction of metabolic networks, etc. Through the above research technologies and strategies, a series of food cell factories are constructed to efficiently produce milk derived key active components, inculding milk active proteins, Human milk oligosaccharides, fat-soluble vitamins and structural fats. At present, some products such as acetylglucosamine, vitamin K2 and phosphatidylserine have been industrialized.

1.Liu Long, Du Guocheng, Liu Yanfeng. Systems and synthetic metabolic engineering (Elsevier press). 2020. ISBN: 978-0-12-821753-5.

2.Liu Long, Chen Jian. Systems and synthetic biotechnology for production of nutraceuticals (Springer-Nature press). 2019. ISBN: 978-981-15-0445-7.

3.Lv Xueqin, Jin Ke, Sun Guoyun, Ledesma-Amaro Rodrigo, Liu Long*. Microscopy imaging of living cells in metabolic engineering.Trends in Biotechnology. 2022. DOI: 10.1016/j.tibtech.2021.10.010.

4.Deng Chen, Wu Yaokang, Lv Xueqin, Li Jianghua, Liu Yanfeng, Du Guocheng, Chen Jian, Liu Long*. Refactoring transcription factors for metabolic engineering.Biotechnology Advances. 2022. 57: 107935.

5.Ling Meixi, Wu Yaokang, Tian Rongzhen, Liu Yanfeng, Yu Wenwen, Tao Guanjun, Li Jianghua, Du Guocheng*, Ledesma-Amaro Rodrigo, Liu Long*. Combinatorial pathway engineering of Bacillus subtilis for production of structurally defined and homogeneous chitooligosaccharides. Metabolic Engineering. 2022. 70: 55-66.

6.Qu Lisha, Xiu Xiang, Sun Guoyun, Zhang Chenyang, Yang Haiquan, Liu Yanfeng, Li Jianghua, Du Guocheng, Lv Xueqin, Liu Long*. Engineered yeast for efficientde novosynthesis of 7-dehydrocholesterol.Biotechnology and Bioengineering. 2022. DOI: org/10.1002/bit.28055.

7.Deng Chen, Lv Xueqin, Li Jianghua, Zhang Hongzhi, Liu Yanfeng, Du Guocheng, Ledesma-Amaro Rodrigo, Liu Long*. Synergistic improvement of N-acetylglucosamine production by engineering transcription factors and balancing redox cofactors.Metabolic Engineering. 2021. 67: 330-346.

8.Xu Xianhao, Li Xueliang, Liu Yanfeng, Zhu Yonglian, Li Jianghua, Du Guocheng, Chen Jian, Ledesma- Amaro Rodrigo, Liu Long*. Pyruvate-responsive genetic circuits for dynamic control of central metabolism.Nature Chemical Biology. 2020. 16: 1261-1268.

9.Wu Yaokang, Chen Taichi, Liu Yanfeng, Tian Rongzhen, Lv Xueqin, Li Jianghua, Du Guocheng, Chen Jian, Ledesma-Amaro Rodrigo, Liu Long*. Design of a programmable biosensor-CRISPRi genetic circuits for dynamic and autonomous dual-control of metabolic flux inB. subtilis.Nucleic Acids Research. 2020. 48: 996-1009.

10.Xu Xianhao, Liu Yanfeng, Du Guocheng, Ledesma-Amaro Rodrigo, Liu Long*. Microbial chassis development for natural products biosynthesis.Trends in Biotechnology. 2020. 38: 779-796.

11.Lv Xueqin, Zhang Cheng, Cui Shixiu, Xu Xianhao, Wang Lingling, Li Jianghua, Du Guocheng, Chen Jian, Ledesma-Amaro Rodrigo, Liu Long*. Assembly of pathway enzymes by rationally engineering functional membrane microdomains for improved N-acetylglucosamine synthesis inBacillus subtilis.Metabolic Engineering. 2020. 61: 96-105.

12.Lv Xueqin, Wu Yaokang, Tian Rongzhen, Gu Yang, Liu Yanfeng, Li Jianghua, Du Guocheng, Ledesma-Amaro Rodrigo, Liu Long*. Synthetic metabolic channel by functional membrane microdomains for compartmentalized flux control.Metabolic Engineering. 2020. 59: 106-118.

13.Dong Xiaomin, Li Nan, Liu Zhenmin, Lv Xueqin, Shen Yu, Li Jianghua, Du Guocheng, Wang Miao*, Liu Long*. CRISPRi-guided multiplexed fine-tuning of metabolic flux for enhanced Lacto-N-neotetraose production inBacillus subtilis.Journal of Agricultural and Food Chemistry, 2020, 68(8): 2477-2484.

14.Deng Jieying, Chen Chunmei, Gu Yang, Lv Xueqin, Liu Yanfeng, Li Jianghua, Ledesma-Amaro Rodrigo, Du Guocheng, Liu Long*. Creating anin vivobifunctional gene expression circuit through an aptamer-based regulatory mechanism for dynamic metabolic engineering inBacillus subtilis.Metabolic Engineering. 2019. 55: 179-190.

15.Gu Yang, Liu Yanfeng, Li Jianghua, Du Guocheng, Ledesma-Amaro Rodrigo, Liu Long*. Synthetic redesign of central carbon and redox metabolism for high yield production of N-acetylglucosamine inBacillus subtilis.Metabolic Engineering. 2019. 51: 59-69.

16.Cui Shixiu, Lv Xueqin, Wu Yaokang, Li Jianghua, Du Guocheng, Ledesma-Amaro Rodrigo, Liu Long*. Engineering a bifunctional Phr60-Rap60-Spo0A quorum-sensing molecular switch for dynamic fine-tuning of menaquinone-7 synthesis inBacillus subtilis.ACS Synthetic Biology. 2019. 8(8): 1826-1837.

17.Deng Jieying, Gu Liuyan, Chen Taichi, Huang Hao, Yin Xiaoqiang, Lv Xueqin, Liu Yanfeng, Li Nan, Liu Zhenmin, Li Jianghua, Du Guocheng, Liu Long*. Engineering the substrate transport and cofactor regeneration systems for enhancing 2’-fucosyllactose synthesis inBacillus subtilis.ACS Synthetic Biology. 2019. 8(10):2418-2427.

18.Wu Yaokang, Chen Taichi, Liu Yenfeng, Lv Xueqin, Li Jianghua, Du Guocheng, Ledesma-Amaro Rodrigo,Liu Long*. CRISPRi allows optimal temporal control of N-acetylglucosamine bioproduction by a dynamic coordination of glucose and xylose metabolism inBacillus subtilis.Metabolic Engineering. 2018. 49: 232-241.

19.Gu Yang, Xu Xianhao, Wu Yaokang, Niu Tengfei, Liu Yanfeng, Li Jianghua, Du Guocheng, Liu Long*. Advances and prospects ofBacillus subtiliscellular factories: from rational design to industrial applications.Metabolic Engineering. 2018. 50: 109-121.

20.Liu Yanfeng, Link Hannes, Liu Long*, Du Guocheng, Chen Jian*, Sauer Uwe. A novel dynamic pathway analysis approach reveals a limiting futile cycle in N-acetylglucosamine overproducingBacilus subtilis.Nature Communications. 2016.7:11933.

Liu Yanfeng, Link Hannes, Liu Long*, Du Guocheng, Chen Jian*, Sauer Uwe. A novel dynamic pathway analysis approach reveals a limiting futile cycle in N-acetylglucosamine overproducingBacillus subtilis.Nature Communications. 2016. 7:11933