Associate Professor

Tel: 86-510-85918307

Email: liusong@jiangnan.edu.cn

Web:

2016 – Present    Associate Professor,   School of Biotechnology, Jiangnan University.

2012 – 2015      Lecture, School   of Biotechnology, Jiangnan University.

2007 – 2011     Ph.D Fellow,   School of Biotechnology, Jiangnan University

2005 – 2007     M.Sc., School   of Food Science and Technology, Jiangnan University

2003 – 2005     Engineer,   Luoniushan Co., Ltd

1999 – 2003        B.Sc., School of Food Science and Technology, Henan University of   Technology

Main research interesting on modification and production of   industrial enzymes, including transglutaminase, lipoxygenase, asparaginase,   pectinase, and etc.

1)   Modification of enzyme application performance

Based on the   investigation on the mechanism of enhanced thermal stability and activity of   lipoxygenase fused with amphipathic peptides, we attempt to design   amphipathic peptides with high specificity or universality to improve the   thermal stabilities and activities of industrial enzymes. We also focus on   mining key loop structures in the enzyme and modifying it for enhanced   catalytic performance through random mutation, sequence replacement, and etc.

2)   Efficient secretory expression of enzymes

To improve the   secretory expression of enzymes, the expression and secretion pathway of   enzyme (gene synthesis, transcription, translation, protein folding and transport,   etc.) in Streptomyces and Aspergillus niger are systematically   analyzed, and the restrictive factors for enzyme production are identified   and subsequently eliminated. In addition, we are interested in the effects of   N-terminal sequence of enzyme on its expression and expression.

Authorized patents:

1.          Song Liu, Yue Feng, Jian Chen, Guocheng DU, Yun   Jiao, Jie Ruan, Hongye Cheng, Hui Gao. Mutant with enhanced secretion of   L-asparaginase and its application. United States Patent 9896678B2

2.          Jian Chen, Guocheng   Du, Xinyao Lu, Song Liu, Juan Zhang. Method for increasing protein   thermal stability. United States Patent 9040274

SCI Papers:

1.            Feng Y, Liu S*,   Jiao Y, Gao H, Wang M, Du G, Chen J*. 2017. Enhanced extracellular production of L-asparaginase   from Bacillus subtilis 168 by B-subtilis WB600 through a   combined strategy. Applied Microbiology and Biotechnology 101:1509-1520.

2.            Lu X, Wang G, Feng Y, Liu   S*, Zhou X, Du G, Chen J. 2016. The N-Terminal alpha-Helix Domain of   Pseudomonas aeruginosa Lipoxygenase Is Required for Its Soluble   Expression in Escherichia coli but Not for Catalysis. Journal of   Microbiology and Biotechnology 26:1701-1707.

3.            Liu S, Wang M, Du G, Chen J. 2016. Improving the active expression of   transglutaminase in Streptomyces lividans by promoter engineering and   codon optimization. BMC Biotechnology 16.

4.            Bi J, Liu S, Du   G, Chen J. 2016. Bile salt   tolerance of Lactococcus lactis is enhanced by expression of bile salt   hydrolase thereby producing less bile acid in the cells. Biotechnology   Letters 38:659-665.

5.            Liu S, Wan D, Wang M, Madzak C, Du G, Chen J. 2015. Overproduction of   pro-transglutaminase from Streptomyces hygroscopicus in Yarrowia   lipolytica and its biochemical characterization. BMC Biotechnology   15.

6.            Liu S, Dai J, Kang Z, Li J, Chen J, Du G. 2015. Production of novel NaN3-resistant   creatine amidinohydrolase in recombinant Escherichia coli (vol 6, pg 248,   2015). Bioengineered 6:248-250.

7.            Xu Z, Liu S*,   Lu X, Rao S, Kang Z, Li J, Wang M, Chen J*. 2014. Thermal inactivation of a recombinant   lipoxygenase from Pseudomonas aeruginosa BBE in the absence and   presence of additives. Journal of the Science of Food and Agriculture 94:1753-1757.

8.            Lu X, Liu S*, Feng Y, Rao S, Zhou X, Wang M, Du G*, Chen J.   2014. Enhanced thermal   stability of Pseudomonas aeruginosa lipoxygenase through modification   of two highly flexible regions. Applied Microbiology and Biotechnology   98:1663-1669.

9.            Lu X, Zhang J, Liu   S, Zhang D, Xu Z, Wu J, Li J, Du G, Chen J. 2013. Overproduction, purification, and characterization   of extracellular lipoxygenase of Pseudomonas aeruginosa in Escherichia   coli. Applied Microbiology and Biotechnology 97:5793-5800.

10.          Lu X, Liu S,   Zhang D, Zhou X, Wang M, Liu Y, Wu J, Du G, Chen J. 2013. Enhanced thermal stability and specific activity   of Pseudomonas aeruginosa lipoxygenase by fusing with self-assembling   amphipathic peptides. Applied Microbiology and Biotechnology 97:9419-9427.

11.          Chen K, Zhang D, Liu   S, Wang NS, Wang M, Du G, Chen J. 2013. Improvement of transglutaminase production by   extending differentiation phase of Streptomyces hygroscopicus:   mechanism and application. Applied Microbiology and Biotechnology 97:7711-7719.

12.          Chen K, Liu S*, Zhang D, Wang M, Shi Z, Du G, Chen J*. 2013. Altered secretary efficiency of Streptomyces   hygroscopicus transglutaminase in Escherichia coli by the   pro-peptide modification. Process Biochemistry 48:782-787.

13.          Chen K, Liu S,   Wang G, Zhang D, Du G, Chen J, Shi Z. 2013. Enhancement of Streptomyces   transglutaminase activity and pro-peptide cleavage efficiency by introducing   linker peptide in the C-terminus of the pro-peptide. Journal of Industrial   Microbiology & Biotechnology 40:317-325.

14.          Yang Y, Zhang D, Liu   S, Jia D, Du G, Chen J. 2012.   Expression and fermentation optimization of oxidized polyvinyl alcohol   hydrolase in E. coli. Journal of Industrial Microbiology &   Biotechnology 39:99-104.

15.          Chen K, Liu S,   Ma J, Zhang D, Shi Z, Du G, Chen J. 2012. Deletion combined with saturation mutagenesis of N-terminal   residues in transglutaminase from Streptomyces hygroscopicus results   in enhanced activity and thermostability. Process Biochemistry 47:2329-2334.

16.          Liu S, Zhang D, Wang M, Cui W, Chen K, Liu Y,   Du G, Chen J, Zhou Z. 2011.   The pro-region of Streptomyces hygroscopicus transglutaminase affects   its secretion by Escherichia coli. FEMS Microbiology Letters   324:98-105.

17.          Liu S, Zhang D, Wang M, Cui W, Chen K, Du G,   Chen J, Zhou Z. 2011. The   order of expression is a key factor in the production of active   transglutaminase in Escherichia coli by co-expression with its pro-peptide.   Microbial Cell Factories 10.

18.          Liu S, Zhang D, Liu L, Wang M, Du G, Chen J. 2010. Enhanced water absorption of wheat   gluten by hydrothermal treatment followed by microbial transglutaminase   reaction. Journal of the Science of Food and Agriculture 90:658-663.

Books:

1.          Jian Chen, Yang Zhu, Song   Liu. Functional Carbohydrates: Development, Characterization, and   Biomanufacture. 2017, CRC Press, ISBN 9781498718776